HotSpot Wizard is a web server for automatic identification of 'hot spots' for engineering of substrate specificity, activity or enantioselectivity of enzymes and for annotation of protein structures. The web server implements the protein engineering protocol, which targets evolutionarily variable amino acid positions located in the active site or lining the access tunnels.
In the output, HotSpot Wizard lists annotated residues ordered by estimated mutability. The results of the analysis are mapped on the enzyme structure and visualized in the web browser using Jmol.
Pavelka, A., Chovancova, E., Damborsky, J. HotSpot Wizard: a Web Server
for Identification of Hot Spots in Protein Engineering, Nucleic Acids
Research 37: W376-W383, 2009.