| Authors: | Jesenska, A., Monincova, M., Chrobakova, T., Hasan, K., Chaloupkova, R., Prokop, Z., Geerlof, A., Damborsky, J. |
| Title: | Isolation and Biochemical Characterization of Haloalkane Dehalogenases DrbA and DmbC: Representatives of Novel Subfamily. |
| Source: | APPLIED AND ENVIRONMENTAL MICROBIOLOGY 75: 5157-5160 2009 |
| Abstract: | |
This study focuses on two representatives of experimentally uncharacterized haloalkane dehalogenases from the subfamily HLD-III. We report biochemical characterization of the expression products of haloalkane dehalogenases genes drbA from Rhodopirellula baltica SH1 and dmbC from Mycobacterium bovis 5033/66. The DrbA and DmbC enzymes show highly oligomeric structure and very low activity with typical substrates of haloalkane dehalogenases. | |
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