Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering

Authors

Prokop, Z., Sato, Y., Brezovsky, J., Mozga, T., Chaloupkova, R., Koudelakova, T., Jerabek, P., Stepankova, V., Natsume, R., Leeuwen, J. G. E., Janssen, D. B., Florian, J., Nagata, Y., Senda, T., Damborsky, J.

Source

ANGEWANDTE CHEMIE INTERNATIONAL EDITION 49: 6111-6115 (2010)

Abstract

In the loop: Engineering of the surface loop in haloalkane dehalogenases affects their enantiodiscrimination behavior. The temperature dependence of the enantioselectivity (lnE versus 1/T) of -bromoalkanes by haloalkane dehalogenases is reversed (red data points) by deletion of the surface loop; the selectivity switches back when an additional single-point mutation is made. This behavior is not observed for -bromoesters.

Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering

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Citation

Prokop, Z., Sato, Y., Brezovsky, J., Mozga, T., Chaloupkova, R., Koudelakova, T., Jerabek, P., Stepankova, V., Natsume, R., Leeuwen, J. G. E., Janssen, D. B., Florian, J., Nagata, Y., Senda, T., Damborsky, J., 2010: Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering. Angewandte Chemie International Edition 49: 6111-6115.

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G. Dankova and A. Kunka awarded Brno Ph.D. Talent
Jaroslav Bendl, Ph.D.: From Loschmidt Laboratories to Mount Sinai School of Medicine in New York
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