Do you need to engineer your protein of interest? Do you wish to employ user-friendly one-stop-shop software tools for the design of mutations and libraries, but do not want to spend time on tedious installations or use the Unix command-line? This interactive course will provide essential theory on different areas of enzyme engineering, and hands-on experience with several software tools suitable for mining new enzymes, engineering the activity, specificity, enantioselectivity, stability, and solubility of enzymes.
You will have the chance to learn from the developers or experienced users of those tools, and discuss the optimal ways to set-up calculations and adequately interpret the results. At the end of this 3-day course, you will be able to design different properties of proteins for your academic research or commercial applications on your own.
Software and databases covered: Aggreprot, AlphaFill, AlphaFold, Caver, CaverDock, Caver Web, ChannelsDB, EnzymeMiner, ESMFold, FireProt, FireProtASR, FireProtDB, HotSpot Wizard, LoopGrafter, ProteinMPNN, RF Diffusion, Solubis, SoluProt and SoluProtMutDB. Our web-tools integrate multiple individual software, and altogether, you will get automated access to more than 50 computational methods and 15 databases.