Engineering a de Novo Transport Tunnel

Here we describe the computational design and directed evolution of a de novo transport tunnel in a haloalkane dehalogenase. Mutants with a blocked native tunnel and a newly opened auxiliary tunnel in a distinct part of the structure showed dramatically modified properties. The mutants with blocked tunnels acquired specificity never observed with native family members: up to 32 times increased substrate inhibition and 17 times reduced catalytic rates. Opening of the auxiliary tunnel resulted in specificity and substrate inhibition similar to those of the native enzyme and the most proficient haloalkane dehalogenase reported to date.


Brezovsky, J., Babkova, P., Degtjarik, O., Fortova, A., Gora, A., Iermak, I., Rezacova, P., Dvorak, P., Kuta Smatanova, I., Prokop, Z., Chaloupkova, R., Damborsky, J., 2016: Engineering a de Novo Transport Tunnel. ACS Catalysis 6: 7597-7610.

Sergio Marques the Most Ardent Journal Reader
Summer School of Protein Engineering
Prof. Donald Hilvert to Give Lectute at Masaryk University
Zbynek Prokop’s Professorship Approved by the President
Open Postdoc Position: Protein Engineering
Ondrej Vavra selected among Southmoravian PhD Talents
Professor Damborsky among Ceska hlava 2017 laureates