Coelenterazine-Utilizing Luciferases with Stable Glow-Type Light Emission, Increased Substrate Affinity, Suppresed Product Inhibition, and Modulated Emission Wavelengths, and Methods of Producing and Using Thereof
Toul, M., Schenkmayerova, A., Marek, M., Pinto, J. G., Planas-Iglesias, J., Pluskal, D., Vasina, M., Bednar, D., Prokop, Z., Damborsky, J.
Masaryk University, Žerotínovo nám. 9, 601 77 Brno, Czech Republic
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The present invention relates to modified protein sequences of coelenterazine-utilizing Renilla-type luciferases exhibiting stable glow-type light emission, high substrate affinity, low product inhibition factor, and modulated emission maximum, and the production and use of thereof as bioluminescent reporters and bioimaging agents.