Coelenterazine-Utilizing Luciferases with Stable Glow-Type Light Emission, Increased Substrate Affinity, Suppresed Product Inhibition, and Modulated Emission Wavelengths, and Methods of Producing and Using Thereof

Authors

Toul, M., Schenkmayerova, A., Marek, M., Pinto, J. G., Planas-Iglesias, J., Pluskal, D., Vasina, M., Bednar, D., Prokop, Z., Damborsky, J.

Applicant

Masaryk University, Žerotínovo nám. 9, 601 77 Brno, Czech Republic

Application number

Date of submission

Date of publication

Abstract

The present invention relates to modified protein sequences of coelenterazine-utilizing Renilla-type luciferases exhibiting stable glow-type light emission, high substrate affinity, low product inhibition factor, and modulated emission maximum, and the production and use of thereof as bioluminescent reporters and bioimaging agents.

Full application EN

Citation

Toul, M., Schenkmayerova, A., Marek, M., Pinto, J. G., Planas-Iglesias, J., Pluskal, D., Vasina, M., Bednar, D., Prokop, Z., Damborsky, J., 2021: Coelenterazine-Utilizing Luciferases with Stable Glow-Type Light Emission, Increased Substrate Affinity, Suppresed Product Inhibition, and Modulated Emission Wavelengths, and Methods of Producing and Using Thereof. Masaryk University, Brno, Czech Republic. Patent XX.

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