A Quantitative Assay of Group E Colicins

Authors

Smarda, J., Damborsky, J.

Source

SCRIPTA MEDICA 64: 111-118 (1991)

Abstract

Two basic methods of quantitative assay of the inhibitive activity of colicins towards bacteria are commonly used in a variety of modifications: the critical dilution spot test and survival count. The former gives the colicin activity in arbitrary units (A.U.), the latter in lethal units (L.U.). In a series of experiments, the two titration methods were compared for all colicins of Group E, namely E1-E9, using Escherichia coli K12-Row indicator bacteria. For all colicins, the L.U. to A.U. ratio equals 2×10^8:1. 1A.U. comprises 2×10^8 L.U. Both advantages and disadvantages, especially the possible error sources, of either colicin titration procedure are briefly discussed. It is emphasized that any colicin titre value match the assay system used.

Full text

Citation

Smarda, J., Damborsky, J., 1991: A Quantitative Assay of Group E Colicins. Scripta Medica 64: 111-118.

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