BenchStab: a Tool for Automated Querying of Web-Based Stability Predictors
Authors
Velecky, J., Berezny, M., Musil, M., Damborsky, J., Bednar, D., Mazurenko, S.
Source
BIOINFORMATICS 40: btae553 (2024)
Abstract
Protein design requires information about how mutations affect protein stability. Many web-based predictors are available for this purpose, yet comparing them or using them en masse is difficult. Here, we present BenchStab, a console tool/Python package for easy and quick execution of 19 predictors and result collection on a list of mutants. Moreover, the tool is easily extensible with additional predictors. We created an independent dataset derived from the FireProtDB and evaluated 24 different prediction methods.