Biochemical Characteristics of the Novel Haloalkane Dehalogenase DatA Isolated from the Plant Pathogen Agrobacterium tumefaciens C58
Hasan, K., Fortova, A., Koudelakova, T., Chaloupkova, R., Ishitsuka, M., Nagata, Y., Damborsky, J., Prokop, Z.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY 77: 1881-1884 (2011)
We report the biochemical characterization of a novel haloalkane dehalogenase DatA isolated from the plant pathogen Agrobacterium tumefaciens C58. DatA possesses a peculiar pair of halide-stabilising residues, Asn-Tyr, which have not been reported to play this role in other known haloalkane dehalogenases. DatA has a number of other unique characteristics, including substrate-dependent and cooperative kinetics, a dimeric structure, and excellent enantioselectivity towards racemic mixtures of chiral brominated alkanes and esters.