Computer-Assisted Engineering of Hyperstable Fibroblast Growth Factor 2

Authors

Dvorak, P., Bednar, D., Vanacek, P., Balek, L., Eiselleova, L., Stepankova, V., Sebestova, E., Kunova Bosakova, M., Konecna, Z., Mazurenko, S., Kunka, A. Vanova, T., Zoufalova, K., Chaloupkova, R., Brezovsky, J., Krejci, P., Prokop, Z., Dvorak, P., Damborsky, J.

Source

BIOTECHNOLOGY AND BIOENGINEERING 115: 850-862 (2018)

Abstract

Fibroblast growth factors (FGFs) serve numerous regulatory functions in complex organisms, and their corresponding therapeutic potential is of growing interest to academics and industrial researchers alike. However, applications of these proteins are limited due to their low stability. Here we tackle this problem using a generalizable computer-assisted protein engineering strategy to create a unique modified FGF2 with nine mutations displaying unprecedented stability and uncompromised biological function. The data from the characterization of stabilized FGF2 showed a remarkable prediction potential of in silico methods and provided insight into the unfolding mechanism of the protein. The molecule holds a considerable promise for stem cell research and medical or pharmaceutical applications.

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Citation

Dvorak, P., Bednar, D., Vanacek, P., Balek, L., Eiselleova, L., Stepankova, V., Sebestova, E., Kunova Bosakova, M., Konecna, Z., Mazurenko, S., Kunka, A. Vanova, T., Zoufalova, K., Chaloupkova, R., Brezovsky, J., Krejci, P., Prokop, Z., Dvorak, P., Damborsky, J., 2018: Computer-Assisted Engineering of Hyperstable Fibroblast Growth Factor 2. Biotechnology and Bioengineering 115: 850-862.

Lecture of Dr. Joan Planas Iglesias
Open Ph.D. Position: Marie Curie Innovative Training Network
Prof. Damborsky to present at an EMBO Workshop
Daniel’s Success in Students` Professional Activities
Three New Masters of Science: Zuzana, Martin and Pavel
Joseph Fourier Prize for Milos
The Dean’s Award for Veronika