Conformational Changes Allow Processing of Bulky Substrates by a Haloalkane Dehalogenase with a Small and Buried Active Site

Authors

Kokkonen, P., Bednar, D., Dockalova, V., Prokop, Z., Damborsky, J.

Source

THE JOURNAL OF BIOLOGICAL CHEMISTRY 29: 11505-11512 (2018)

Abstract

Haloalkane dehalogenases catalyze the hydrolysis of halogen–carbon bonds in organic halogenated compounds and as such are of great utility as biocatalysts. The crystal structures of the haloalkane dehalogenase DhlA from the bacterium from Xanthobacter autotrophicus GJ10, specifically adapted for the conversion of the small 1,2-dichloroethane (DCE) molecule, display the smallest catalytic site (110 Å3) within this enzyme family. However, during a substrate-specificity screening, we noted that DhlA can catalyze the conversion of far bulkier substrates, such as the 4-(bromomethyl)-6,7-dimethoxy-coumarin (220 Å3). This large substrate cannot bind to DhlA without conformational alterations. These conformational changes have been previously inferred from kinetic analysis, but their structural basis has not been understood. Using molecular dynamic simulations, we demonstrate here the intrinsic flexibility of part of the cap domain that allows DhlA to accommodate bulky substrates. The simulations displayed two routes for transport of substrates to the active site, one of which requires the conformational change and which is likely the route for bulky substrates. These results provide insights into the structure–dynamics–function relationships in enzymes with deeply buried active sites. Moreover, understanding the structural basis for the molecular adaptation of DhlA to DCE introduced into the biosphere during the industrial revolution provides a valuable lesson in enzyme design by nature.

Full text •  Supplement

Citation

Kokkonen, P., Bednar, D., Dockalova, V., Prokop, Z., Damborsky, J., 2018: Conformational Changes Allow Processing of Bulky Substrates by a Haloalkane Dehalogenase with a Small and Buried Active Site. The Journal of Biological Chemistry 29: 11505-11512.

How LL Research Focus Fits the Big Issues in Medicine
Open Postdoctoral Position: Protein Structural Biologist
Martin Marek’s Success in GAMU Project
Pleased to Announce a New Successful PhD Candidate: Tom Buryska
Congratulations to Piia and Colleagues on Their JACS Publication!
Woman Power Growing Stronger in LL: Lucinka
A New Addition to LL Team: Zanetka