DspA from Strongylocentrotus purpuratus: the First Biochemically Characterized Haloalkane Dehalogenase of Non-Microbial Origin

Authors

Fortova, A., Sebestova, E., Stepankova, V., Koudelakova, T., Palkova, L., Damborsky, J., Chaloupkova, R.

Source

BIOCHIMIE 95: 2091-2096 (2013)

Abstract

Haloalkane dehalogenases are known as bacterial enzymes cleaving a carbon-halogen bond in halogenated compounds. Here we report the first biochemically characterized non-microbial haloalkane dehalogenase DspA from Strongylocentrotus purpuratus. The enzyme shows a preference for terminally brominated hydrocarbons and enantioselectivity towards β-brominated alkanes. Moreover, we identified other putative dehalogenases of eukaryotic origin, representing targets for future experiments to discover dehalogenases with novel catalytic properties.

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Citation

Fortova, A., Sebestova, E., Stepankova, V., Koudelakova, T., Palkova, L., Damborsky, J., Chaloupkova, R., 2013: DspA from Strongylocentrotus purpuratus: the First Biochemically Characterized Haloalkane Dehalogenase of Non-Microbial Origin. Biochimie 95: 2091-2096.

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