DspA from Strongylocentrotus purpuratus: the First Biochemically Characterized Haloalkane Dehalogenase of Non-Microbial Origin

Authors

Fortova, A., Sebestova, E., Stepankova, V., Koudelakova, T., Palkova, L., Damborsky, J., Chaloupkova, R.

Source

BIOCHIMIE 95: 2091-2096 (2013)

Abstract

Haloalkane dehalogenases are known as bacterial enzymes cleaving a carbon-halogen bond in halogenated compounds. Here we report the first biochemically characterized non-microbial haloalkane dehalogenase DspA from Strongylocentrotus purpuratus. The enzyme shows a preference for terminally brominated hydrocarbons and enantioselectivity towards β-brominated alkanes. Moreover, we identified other putative dehalogenases of eukaryotic origin, representing targets for future experiments to discover dehalogenases with novel catalytic properties.

Full text

Citation

Fortova, A., Sebestova, E., Stepankova, V., Koudelakova, T., Palkova, L., Damborsky, J., Chaloupkova, R., 2013: DspA from Strongylocentrotus purpuratus: the First Biochemically Characterized Haloalkane Dehalogenase of Non-Microbial Origin. Biochimie 95: 2091-2096.

Gaspar a Gaussian Expert
1st Hands-on Computational Enzyme Design Workshop
Marie Curie Fellowship for Dmitri
Rayyan Announced as Best Presenter
Researchers’ Night 2019 in LL
Open Postdoctoral Position: Protein Structural Biologist
Andrea Awarded for Excellent Research