Effect of the Carbon Source on Assessment of Degrading Bacteria with the Spread-Plating Technique During In Situ Bioremediation
Damborsky, J., Damborska, M., Stipek, S., Jesenska, A., Trantirek, L., Sklenar, V.
FOLIA MICROBIOLOGICA 45: 35-40 (2000)
Spread-plating belongs to traditional microbiological methods employed for quantification of subsurface microilora during bioremediation projects in the Czechia. Concentration of degrading organisms is estimated from the number of colonies grown on agar p lates supplied with contaminant as the sole carbon source. The data obtained during in situ bioremediation of the Dacice site contaminated by cutting oil suggests that changes in the composition of the carbon source in the subsurface may cause a discrepancy between laboratory data and situation in subsurface.