Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering

Authors

Prokop, Z., Sato, Y., Brezovsky, J., Mozga, T., Chaloupkova, R., Koudelakova, T., Jerabek, P., Stepankova, V., Natsume, R., Leeuwen, J. G. E., Janssen, D. B., Florian, J., Nagata, Y., Senda, T., Damborsky, J.

Source

ANGEWANDTE CHEMIE INTERNATIONAL EDITION 49: 6111-6115 (2010)

Abstract

In the loop: Engineering of the surface loop in haloalkane dehalogenases affects their enantiodiscrimination behavior. The temperature dependence of the enantioselectivity (lnE versus 1/T) of -bromoalkanes by haloalkane dehalogenases is reversed (red data points) by deletion of the surface loop; the selectivity switches back when an additional single-point mutation is made. This behavior is not observed for -bromoesters.

Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering

Full text •  Supplement

Citation

Prokop, Z., Sato, Y., Brezovsky, J., Mozga, T., Chaloupkova, R., Koudelakova, T., Jerabek, P., Stepankova, V., Natsume, R., Leeuwen, J. G. E., Janssen, D. B., Florian, J., Nagata, Y., Senda, T., Damborsky, J., 2010: Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering. Angewandte Chemie International Edition 49: 6111-6115.

David to Participate in NGSchool2022
Stas to Present at AI for Enzyme Instructed Synthetic Catalysis
David to Present at XXV Biological Days
1st Droplet Sorting in LL
Jiri to Present at Business2Science
Prof. Bornscheuer to Give Lecture at MU
Dean’s Award for Pavel