Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering

Authors

Prokop, Z., Sato, Y., Brezovsky, J., Mozga, T., Chaloupkova, R., Koudelakova, T., Jerabek, P., Stepankova, V., Natsume, R., Leeuwen, J. G. E., Janssen, D. B., Florian, J., Nagata, Y., Senda, T., Damborsky, J.

Source

ANGEWANDTE CHEMIE INTERNATIONAL EDITION 49: 6111-6115 (2010)

Abstract

In the loop: Engineering of the surface loop in haloalkane dehalogenases affects their enantiodiscrimination behavior. The temperature dependence of the enantioselectivity (lnE versus 1/T) of -bromoalkanes by haloalkane dehalogenases is reversed (red data points) by deletion of the surface loop; the selectivity switches back when an additional single-point mutation is made. This behavior is not observed for -bromoesters.

Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering

Full text •  Supplement

Citation

Prokop, Z., Sato, Y., Brezovsky, J., Mozga, T., Chaloupkova, R., Koudelakova, T., Jerabek, P., Stepankova, V., Natsume, R., Leeuwen, J. G. E., Janssen, D. B., Florian, J., Nagata, Y., Senda, T., Damborsky, J., 2010: Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering. Angewandte Chemie International Edition 49: 6111-6115.

Celebration of Achievements
Loschmidt Labs Got ELEGANCE Grant
Anthony to Present at the 15th European Biophysics Congress
Zbynek at Kinetics 2025
Kaja and Martin Went to Synchrotron
Daniel Nominated for the Government Prize for Talented Students
Spring Trip to “Kamenny vrch”