FireProt: Web Server for Automated Design of Thermostable Proteins

Authors

Musil, M., Stourac, J., Bendl, J., Brezovský, J., Prokop, Z., Zendulka, J., Martinek, T., Bednar, D., Damborsky, J.

Source

NUCLEIC ACIDS RESEARCH 45 (W1): W393-W399 (2017)

Abstract

There is a continuous interest in increasing proteins stability to enhance their usability in numerous biomedical and biotechnological applications. A number of in silico tools for the prediction of the effect of mutations on protein stability have been developed recently. However, only single-point mutations with a small effect on protein stability are typically predicted with the existing tools and have to be followed by laborious protein expression, purification, and characterization. Here, we present FireProt, a web server for the automated design of multiple-point thermostable mutant proteins that combines structural and evolutionary information in its calculation core. FireProt utilizes sixteen tools and three protein engineering strategies for making reliable protein designs. The server is complemented with interactive, easy-to-use interface that allows users to directly analyze and optionally modify designed thermostable mutants. FireProt is freely available at http://loschmidt.chemi.muni.cz/fireprot.

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Citation

Musil, M., Stourac, J., Bendl, J., Brezovský, J., Prokop, Z., Zendulka, J., Martinek, T., Bednar, D., Damborsky, J., 2017: FireProt: Web Server for Automated Design of Thermostable Proteins. Nucleic Acids Research 45 (W1): W393-W399.

Lecture of Dr. Joan Planas Iglesias
Open Ph.D. Position: Marie Curie Innovative Training Network
Prof. Damborsky to present at an EMBO Workshop
Daniel’s Success in Students` Professional Activities
Three New Masters of Science: Zuzana, Martin and Pavel
Joseph Fourier Prize for Milos
The Dean’s Award for Veronika