Novosphingobium barchaimii sp. nov., Isolated from a Hexachlorocyclohexane (HCH) Contaminated Soil
Niharika, N., Moskalikova, H., Kaur, J., Sedlackova, M., Hampl, A., Damborsky, J., Prokop, Z., Lal, R.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 63: 667-672 (2013)
A yellow pigmented bacterial strain, designated LL02T was isolated from HCH contaminated soil from Spolana, Neratovice former Czech producer of Lindane. A neighbour-joining tree based on 16S rRNA gene sequences showed that strain LL02T occupied a distinct phylogenetic position in the Novophingobium cluster, showing the highest similarity with Novosphingobium resinovorum NCIMB 8767T (98.59%). The DNA-DNA relatedness values of strain LL02T with its phylogenetic neighbours were found to be less than 70% indicating that strain LL02T represents a novel species of the genus Novosphingobium. The DNA G+C content of strain LL02T was 67.72±0 %. The major respiratory quinone present was ubiquinone Q10. The polar lipid profile of the strain LL02T also corresponded to those reported for other Novosphingobium species (phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylmonomethylethanolamine and sphingoglycolipids), thus supporting its identification as a member of the genus Novosphingobium. Spermidine was the major polyamine observed in the cell. The major fatty acids are summed feature 3 (consisting of 16:1ω6c and/or 16:1ω7c; 40.13 %) and summed feature 8 (consisting of 18:1 ω7c / and/or 18:1 ω6c, 31.09 %) and 14:0 2-OH (23.16%) was the major hydroxyl fatty acid. The results obtained after DNA-DNA hybridization, biochemical and physiological tests clearly distinguished the strain from closely related species of the genus Novosphingobium. Thus, the strain LL02T represents a novel species of the genus Novosphingobium for which the name Novosphingobium barchaimii LL02T sp. nov. is proposed (CCM= 7980T, DSMZ =25411T).