Protection of Arabidopsis Blunt-Ended Telomeres is Mediated by a Physical Association with the Ku Heterodimer

Authors

Valuchova, S., Fulnecek, J., Prokop, Z., Stolt-Bergner, P., Janouskova, E., Hofr, C., Riha, K.

Source

THE PLANT CELL ONLINE 29: 1533-1545 (2017)

Abstract

Telomeres form specialized chromatin that protects natural chromosome termini from being recognized as DNA double-strand breaks. Plants possess unusual blunt-ended telomeres that are unable to form t-loops or complex with single-strand DNA binding proteins, raising the question of the mechanism behind their protection. We have previously suggested that blunt-ended telomeres in Arabidopsis thaliana are protected by Ku, a DNA repair factor with a high affinity for DNA ends. In non-homologous end joining (NHEJ), Ku loads onto broken DNA via a channel consisting of positively charged amino acids. Here, we demonstrate that while association of Ku with plant telomeres also depends on this channel, Ku’s requirements for DNA binding differ between DNA repair and telomere protection. We show that a Ku complex proficient in DNA loading but impaired in translocation along DNA is able to protect blunt-ended telomeres but is deficient in DNA repair. This suggests that Ku physically sequesters blunt-ended telomeres within its DNA binding channel, shielding them from other DNA repair machineries.

Full text

Citation

Valuchova, S., Fulnecek, J., Prokop, Z., Stolt-Bergner, P., Janouskova, E., Hofr, C., Riha, K., 2017: Protection of Arabidopsis Blunt-Ended Telomeres is Mediated by a Physical Association with the Ku Heterodimer. The Plant Cell Online 29: 1533-1545.

Ondrej Vavra celebrating success with his presentation on CaverDock
Lecture by Dr. Stavrakis from ETH Zurich
High evaluation of the team’s research performance
Klaudia Chmelova and Veronika Liskova passed doctoral state exam
ELIXIR CZ annual conference 15 – 16 Nov, 2017
New video explaining the goals of Rafts4Biotech EU project
Invitation to a presentation by Synthetic Genomics