Site-specific Analysis of Protein Hydration Based on Unnatural Amino Acid Fluorescence

Authors

Amaro, M., Brezovsky, J., Kovacova, S., Sykora, J., Bednar, D., Nemec, V., Liskova, V., Kurumbang, N., Beerens, K., Chaloupkova, R., Paruch, K., Hof, M., Damborsky, J.

Source

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 137: 4988-4992 (2015)

Abstract

Hydration of proteins profoundly affects their functions. We describe a simple and general method for site-specific analysis of protein hydration based on the in vivo incorporation of fluorescent unnatural amino acids and their analysis by steady-state fluorescence spectroscopy. Using this method, we investigate the hydration of functionally important regions of dehalogenases. The experimental results are compared to findings from molecular dynamics simulations.

Full text •  Supplement

Citation

Amaro, M., Brezovsky, J., Kovacova, S., Sykora, J., Bednar, D., Nemec, V., Liskova, V., Kurumbang, N., Beerens, K., Chaloupkova, R., Paruch, K., Hof, M., Damborsky, J., 2015: Site-specific Analysis of Protein Hydration Based on Unnatural Amino Acid Fluorescence. Journal of the American Chemical Society 137: 4988-4992.

Pleased to Announce a New Successful PhD Candidate: Tom Buryska
Congratulations to Piia and colleagues on their JACS publication!
Woman Power Growing Stronger in LL: Lucinka
A New Addition to LL Team: Zanetka
Open Position: Laboratory Technician
A New Addition to LL Team: Maruska
Lecture of Dr. Joan Planas Iglesias