Site-specific Analysis of Protein Hydration Based on Unnatural Amino Acid Fluorescence

Authors

Amaro, M., Brezovsky, J., Kovacova, S., Sykora, J., Bednar, D., Nemec, V., Liskova, V., Kurumbang, N., Beerens, K., Chaloupkova, R., Paruch, K., Hof, M., Damborsky, J.

Source

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 137: 4988-4992 (2015)

Abstract

Hydration of proteins profoundly affects their functions. We describe a simple and general method for site-specific analysis of protein hydration based on the in vivo incorporation of fluorescent unnatural amino acids and their analysis by steady-state fluorescence spectroscopy. Using this method, we investigate the hydration of functionally important regions of dehalogenases. The experimental results are compared to findings from molecular dynamics simulations.

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Citation

Amaro, M., Brezovsky, J., Kovacova, S., Sykora, J., Bednar, D., Nemec, V., Liskova, V., Kurumbang, N., Beerens, K., Chaloupkova, R., Paruch, K., Hof, M., Damborsky, J., 2015: Site-specific Analysis of Protein Hydration Based on Unnatural Amino Acid Fluorescence. Journal of the American Chemical Society 137: 4988-4992.

Martin Toul Awarded Rector’s Award
Seminar with Prof. Ing. Stepan Podzimek from SYNPO, a.s.
Success at Vernadsky Contest for Jan Cesnek
Interview with Profs. Damborsky and Prokop
Prof. Donald Hilvert to Give Lectute at Masaryk University
New Postdoc Position for Bryja & Damborsky Labs
Prof. deMello to Give Lecture at MU