Sphingobium baderi sp. nov., Isolated from a Hexachlorocyclohexane (HCH) Dump Site in Spolana

Authors

Kaur, J., Moskalikova, H., Niharika, N., Sedlackova, M., Hampl, A., Damborsky, J., Prokop, Z., Lal, R.

Source

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 63: 723-728 (2013)

Abstract

A gram negative, rod-shaped and white coloured bacterial strain designated LL03T, was isolated from hexachlorocyclohexane-contaminated soil in Spolana Neratovice, former Czech producer of Lindane. The strain LL03T was found to be a degrader of α-, γ- and δ- isomers of Hexachlorocyclohexane while no significant degradation activity was observed for β-isomer. A neighbour-joining tree based on 16S rRNA gene sequences showed that strain LL03T occupied a distinct phylogenetic position in the Sphingobium cluster, showing the highest similarity with Sphingobium wenxiniae CC-FH12-1 (99.2%). The DNA G+C content of strain LL03T was 67.0 mol %. The DNA-DNA relatedness values of the strain LL03T with its close phylogenetic neighbours were below the threshold level of 70 %, supporting its identification as a novel species of the genus Sphingobium. The predominant respiratory quinone was ubiquonone Q10. The polar lipid profile of the strain LL03T also corresponded to those reported for other Sphingobium species (phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylmonomethylethanolamine and sphingoglycolipid) supporting its identification as a member of the genus Sphingobium. Spermidine was identified as the major polyamine. The predominant fatty acids were 16:0 (14.3 %), 16:1 ω7c/16:1 ω6c (21.8 %), 18:1 ω7c/18:1 ω6c (12.1 %) and 14:0 2OH (8.4 %). The polar lipid pattern, presence of spermidine and ubiquinone Q10, the predominance of the cellular fatty acids C18:1ω7c, C16:0 and C14:0 2-OH and the G+C content of the genomic DNA supported the affiliation of the strain to the genus Sphingobium. The results obtained after DNA-DNA hybridization, biochemical and physiological tests clearly distinguished it from closely related species of the genus Sphingobium. Therefore, the strain LL03T represents a novel species of the genus Sphingobium for which the name Sphingobium baderi LL03T sp. nov. is proposed (=CCM 7981(T ) = DSMZ 25433 (T )).

Full text

Citation

Kaur, J., Moskalikova, H., Niharika, N., Sedlackova, M., Hampl, A., Damborsky, J., Prokop, Z., Lal, R., 2013: Sphingobium baderi sp. nov., Isolated from a Hexachlorocyclohexane (HCH) Dump Site in Spolana. International Journal of Systematic and Evolutionary Microbiology 63: 723-728.

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