Biotransformation of Chloro- and Bromoalkenes by Crude Extracts of Rhodococcus erythropolis Y2
Authors
Damborsky, J., Manova, K., Berglund, A., Sjostrom, M. and Nemec, M.
Source
ADVANCES IN ENVIRONMENTAL RESEARCH 1: 50-57 (1997)
Abstract
Dehalogenation of chlorinated and brominated alkenes by the crude extract of Rhodococcus erythropolis Y2 has been studied in order to generate suitable data for future development of Quantitative Structure-Biodegradability Relationships (QSBRs). Compounds for testing were selected from a group of 83 chloro- and bromoalkenes using a Principal Component Analysis. Nine compounds out of 19 tested were shown to undergo dehalogenation by crude extracts of the Y2 strain. Preliminary (qualitative) investigation reveals that the extract of Rhodococcus erythropolis Y2 does not attack compounds with carbon atoms bearing more than one halogen substituent. Short-chain chlorinated compounds, recalcitrant in saturated form, were easily dehalogenated upon “obtaining” of the unsaturated bond, while the opposite has been observed for brominated compounds – saturated ones are relatively good substrates compared to unsaturated ones. A sufficient amount of data has been obtained in this study to perform QSBRs analysis, and quantitatively relate the structure of the tested substrates to their dehalogenation rates.