Computational Tools for Designing and Engineering Enzymes

Authors

Damborsky, J., Brezovsky, J.

Source

CURRENT OPINION IN CHEMICAL BIOLOGY 19: 8-16 (2014)

Abstract

Protein engineering strategies aimed at constructing enzymes with novel or improved activities, specificities, and stabilities greatly benefit from in silico methods. Computational methods can be principally grouped into three main categories: (i) bioinformatics, (ii) molecular modelling, and (iii) de novo design. Particularly de novo protein design is experiencing rapid development, resulting in more robust and reliable predictions. A recent trend in the field is to combine several computational approaches in an interactive manner and to complement them with structural analysis and directed evolution. A detailed investigation of designed catalysts provides valuable information on the structural basis of molecular recognition, biochemical catalysis, and natural protein evolution.

Full text

Citation

Damborsky, J., Brezovsky, J., 2014: Computational Tools for Designing and Engineering Enzymes. Current Opinion in Chemical Biology 19: 8-16.

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