Coelenterazine-Utilizing Luciferases with Stable Glow-Type Light Emission, Increased Substrate Affinity, Suppresed Product Inhibition, and Modulated Emission Wavelengths, and Methods of Producing and Using Thereof
Authors
Toul, M., Smith, A., Marek, M., Pinto, J. G., Planas-Iglesias, J., Pluskal, D., Vasina, M., Bednar, D., Prokop, Z., Damborsky, J.
Applicant
Masaryk University, Žerotínovo nám. 9, 601 77 Brno, Czech Republic
Application number
WO/2023/021023
Date of submission
2021-08-16
Date of publication
2023-02-23
Abstract
The present invention relates to modified protein sequences of coelenterazine-utilizing Renilla-type luciferases exhibiting stable glow-type light emission, high substrate affinity, low product inhibition factor, and modulated emission maximum, and the production and use of thereof as bioluminescent reporters and bioimaging agents.